Adhesion Protein Protocols

The second edition of Adhesion Protein Protocols combines traditionaltechniques with cutting-edge and novel techniques that can be adaptedeasily to different molecules and cell types. The topics discussed inthis updated second edition include novel techniques for studyingcell-cell adhesion, neutrophil chemotaxis, in vitro assays used tostudy leukocyte migration through monolayers of cultured endothelialcells, and novel techniques to purify pseudopodia from migratory cells.This book also discusses the study of cell-matrix interaction, RNAinterference, fluorescence recovery after photobleaching, actinpurification, the application of microarray techniques, and the role ofadhesion proteins in the study of proteomics.The protocols discussed in this volume are suitable for both novice andexpert scientists, who will gain further insight into the complex andincompletely understood processes involved in cellular adhesion.